Compendium Of In Vivo Monitoring In Real-time Molecular Neuroscience - Volume 2: Microdialysis And Sensing Of Neural Tissues

Volume 1 of this series focused mainly on small molecules which are intrinsically electroactive. Volume 2 continues to provide current approaches to real time measurements of essential species in the central nervous system. It describes microdialysis, a sampling technique, which facilitates the removal of small volumes of solution containing the analyte(s) of interest which can be subsequently separated by chromatography and analyzed by a range of techniques. These include electrochemistry, UV-Vis spectroscopy, fluorescence and mass spectroscopy. This approach affords the opportunity to measure a range of targets in real-time thus providing access to pharmacokinetic/pharmacodynamics measurements affording the possibility of measuring the disappearance of a drug after administration and the subsequent appearance of metabolites. Microdialysis further affords the ability to sample peptides and proteins of neurobiological interest. Consistent with the goals of Volume 1, Volume 2 seeks to provide for scientists in the field of neurobiology a base of bioanalytical techniques that may not be familiar to them. Specific examples are provided and the fundamental basis of the technique is also covered.Contents: Separation-Based Methods Combined with Microdialysis Sampling for Monitoring Neurotransmitters and Drug Delivery to the Brain (Rachel A Saylor, Sara R Thomas and Susan M Lunte) Microdialysis for Pharmacokinetic and Pharmacodynamic Studies with Focus on the CNS (Margareta Hammarlund-Udenaes) Real-Time Neurochemical Monitoring Using Microdialysis (Michelle L Rogers and Martyn G Boutelle) In Vivo Brain Microdialysis in Human Subjects During Cognitive Tests (Robert J Buchanan, Conor M Gerace and Klevest Gjini) Advanced Microdialysis Approaches Resolve Differences in Serotonin Homeostasis and Signaling (Maureen M Sampson, Hongyan Yang and Anne M Andrews) Improving Temporal Resolution in Online Microdialysis/Liquid Chromatography (Jing Zhang, Khanh T Ngo, Stephen R Groskreutz and Stephen G Weber) Liquid Chromatography-Mass Spectrometry Analysis of Dialysate and Applications with Selective Neuronal Stimulation (Jenny-Marie T Wong, Samuel S Pappas and Robert T Kennedy) Derivatization Chemistries for Improved Detection of Monoamine Neurotransmitters and Their Metabolites in Microdialysis Samples by Liquid Chromatography with Fluorescence Detection and Tandem Mass Spectrometry (Jan Kehr and Takashi Yoshitake) Microdialysis Contributions to the Understanding of Reward and Addiction (Roy A Wise and Zhi-Bing You) Voltammetry Reveals How Mesolimbic Dopamine Relates to Instrumental Reward Seeking (Kate M Wassum) In Vivo Neurochemical Monitoring of the Crab Cancer Borealis via Microdialysis and Mass Spctrometry (Zhidan Liang and Lingiun Li) In Vivo Microdialysis Coupled with Selective Online Electrochemical Systems to Study Ascorbate Dynamics in the Rat Brain (Meining Zhang, Fei Wu and Langqun Mao) Open Volume Microfluidic Probes (Gavin D M Jeffries, Anna A Kim, Kiryl Kustanovich and Aldo Jesorka) Push–Pull Perfusion Sampling of Thin Neuronal Tissues (Scott A Shippy and Geovannie Ojeda-Torres) Microdialysis Flux Considerations (Julie A Stenken and Samuel L Patton) Advancing Intracranial Microdialysis Sampling and Measurements (Andrea Jaquins-Gerstl, Erika L Varner, Kathryn M Nesbitt and Adrian C Michael) Readership: Students and researchers in biochemistry, neurobiology, neuroanatomy, biomedical sciences and bioengineering. Microdialysis;Microfluidics;Sensors;Neurotransmitters;Pharmacokinetics;Pharmacodynamics;Traumatic Brain Injury;UHPLC;LC-MSKey Features: Excellent balance between the development and validation of methodology combined with investigators focused on detailed understanding of neurobiological processes