A Protocol for Digesting Internal Soft Tissues and Mounting Spiders for Scanning Electron Microscopy (Short Communication) (Report) A Protocol for Digesting Internal Soft Tissues and Mounting Spiders for Scanning Electron Microscopy (Short Communication) (Report)

A Protocol for Digesting Internal Soft Tissues and Mounting Spiders for Scanning Electron Microscopy (Short Communication) (Report‪)‬

The Journal of Arachnology 2007, Sept, 35, 3

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Publisher Description

ABSTRACT. We describe a simple protocol for digesting the internal soft tissues of spiders using an enzyme complex known as pancreatin. This technique is preferred over digestions with caustic agents because it better preserves the cuticle surface, allowing its study by means of scanning electron or transmitted light microscopy. In addition, we describe a technique for mounting spider body parts for scanning electron microscopy using an acryloid polymer. Keywords: Digestion, pancreatin, dissection, SEM

GENRE
Science & Nature
RELEASED
2007
1 September
LANGUAGE
EN
English
LENGTH
8
Pages
PUBLISHER
The American Arachnological Society
SIZE
252
KB

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