Extracellular Vesicles

This volume presents the latest advancements in extracellular vesicles (EV) research, drawing on the expertise of investigators across disciplines and institutions. The chapter in this book cover a wide array of experimental approaches, from foundational isolation and enrichment techniques to cutting-edge analytical tools and therapeutic applications. Chapter topics include tangential flow filtration for large-scale isolation, magnetic bead-based separation, and optimized methods for recovering EVs from diverse biological sources. Some chapter also cover specialized procedures that detail the capture of EV surface proteins, electron tomography-based structural analysis, and innovative strategies to load small RNAs into EVs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Extracellular Vesicles serves as a practical and accessible reference for both new and experienced researchers. Whether working with clinical samples, developing therapeutic EVs, or studying their basic biology, investigators will find detailed protocols accompanied by critical commentary on experimental design, controls, and interpretation.