Molecular Biology

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented.

Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.



- Includes a glossary of over 200 terms important for understanding molecular biology



- Uses an inexpensive source of eukaryotic cells - great for schools on a budget



- Includes Methods Locator that provides instant access to the latest methods



- Contain clearly written, easy-to-follow, student-tested instructions:



- Sterile techniques



- Phage titration



- Gel electrophoresis of DNA



- Restriction enzyme digestion



- Plasmid isolation



- Transformation of E. Coli



- Recombinant DNA cloning



- Nick translation labeling



- Nonradioactive primer labelling



- Nonradioactive DNA detection



- Southern blotting



- Colony hybridization



- Purification of plant DNA



- RNA purification



- Northern blotting



- Purification of poly A+ RNA



- Polymerase chain reaction (PCR)