Comparative Efficacy of Pcr-Based Restriction Fragment Length Polymorphism (RFLP) & Multiplex PCR for Glycoprotein B (Gb) Genotyping of Human Cytomegalovirus (Polymerase Chain Reaction) (Report)
Indian Journal of Medical Research 2007, August, 126, 2
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- 2,99 €
Beschreibung des Verlags
Background & objectives: Glycoprotein B (gB), involved in ceil-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. Methods: The PCR based RFLP and the multiplex nested PCR were applied on standard strain of HCMV AD169, 4 clinical HCMV isolates and 70 clinical specimens positive for HCMV by pp65 antigenaemia assay or nested PCR for mtr II region or both.
GENRE
Wissenschaft und Natur
ERSCHIENEN
2007
1. August
SPRACHE
EN
Englisch
UMFANG
14
Seiten
VERLAG
Indian Council of Medical Research
ANBIETERINFO
The Gale Group, Inc., a Delaware corporation and an affiliate of Cengage Learning, Inc.
GRÖSSE
231,4
kB
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