An exhaustive guide to assay design for quantitative real-time PCR. The book describes the basic concepts important for amplicon selection and primer and probe design. There are step-by-step examples for designing probe-based and SYBR Green assays targeting mRNA and fungal pathogens using several popular design programs. These are then exposed to extensive in silico analysis to identify the optimum amplicon/primer/probe combination. There is a detailed trouble shooting guide, a listing of instruments, reagents and additional information available on the internet, all with hyperlinks. In addition, there are three Keynote presentations summarising the main concepts of standard assay design, multiplex assay design and explaining the rationale behind MIQE, the guidelines for qPCR publication transparency.
Customer ReviewsSee All
Dr Jim F Huggett
What a terrific idea.
This detailed, affordable iBook is ideal for those who are new to qPCR or who have been performing the technique, but who are having problems and/or are not sure how best to assess their findings. This is £5.99 well spent and can only assist you in your endeavors.
And of course it is written in the clear terse language so characteristic of Professor Bustin.
A most welcome text that I heartily recommend.