Enrichment of Tetranucleotide Microsatellite Loci from Invertebrate Species‪.‬

ABSTRACT An experimental procedure using biotin labelled probes and streptavidin bound magnetic beads was developed to produce microsatellite-enriched libraries in two bivalve species, geoduck and Japanese scallop, and one crustacean, Dungeness crab. Microsatellite libraries were enriched tar GAT[A.sub.4] and GAC[A.sub.4] by 75 84%. The magnetic bead protocol produced genomic inserts ranging in size from 347-449 up. with an average 5' and 3' flanking of 89-130 bp. Eight polymorphic loci were isolated from both geoduck dam and Dungeness crab. The heterozygosities ranged from 0.57-0.92 for Dungeness crab and 0.91-0.97 for geoduck. These novel polymorphic microsatellite loci will be useful in studying recruitment dynamics and genetic structure. KEY WORDS: microsatellites, enrichment, magnetic beads, geoduck, crab, scallop