Enrichment of Tetranucleotide Microsatellite Loci from Invertebrate Species. Enrichment of Tetranucleotide Microsatellite Loci from Invertebrate Species.

Enrichment of Tetranucleotide Microsatellite Loci from Invertebrate Species‪.‬

Journal of Shellfish Research 2004, August, 23, 2

Journal of Shellfish Research
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Publisher Description

ABSTRACT An experimental procedure using biotin labelled probes and streptavidin bound magnetic beads was developed to produce microsatellite-enriched libraries in two bivalve species, geoduck and Japanese scallop, and one crustacean, Dungeness crab. Microsatellite libraries were enriched tar GAT[A.sub.4] and GAC[A.sub.4] by 75 84%. The magnetic bead protocol produced genomic inserts ranging in size from 347-449 up. with an average 5' and 3' flanking of 89-130 bp. Eight polymorphic loci were isolated from both geoduck dam and Dungeness crab. The heterozygosities ranged from 0.57-0.92 for Dungeness crab and 0.91-0.97 for geoduck. These novel polymorphic microsatellite loci will be useful in studying recruitment dynamics and genetic structure. KEY WORDS: microsatellites, enrichment, magnetic beads, geoduck, crab, scallop

GENRE
Science & Nature
RELEASED
2004
1 August
LANGUAGE
EN
English
LENGTH
23
Pages
PUBLISHER
National Shellfisheries Association, Inc.
SIZE
202.4
KB

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