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Nanobacteria (calcifying nanoparticles, nanobes, nanobacterium) are mysterious particles that have spurred one of the biggest controversies in modern microbiology. Nanobacteria were discovered as cell culture contaminants more than 25 years ago. They are not members of Eubacteria or Archaea, but form an entity of their own that may be of primordial origin. Nevertheless, the literature and evidence on the role of nanobacteria in oral diseases is at a rather hypothetical stage with scarce studies both in-vitro and in-vivo to culminate sufficient reliable evidence. At this mini-book we will try to introduce nanobacteria to dental practitioners and discuss their role in oral diseases.