Alabama Academy of Science Symposium, 2006 "Southeastern Society for Environmental Toxicology and Chemistry" Annual Meeting, Troy University (Conference Notes‪)‬

THE EVALUATION OF CRYO-PROTECTION ON EMBRYOS OF XENUPUS LARVA. Marie Pierre-Louis, J.S.U., Jacksonville, AL 36265. Dr. Cline, Dept of Biology, J.S.U., Jacksonville, AL 36265. The use of cryo-preserving embryos of Amphibians could be useful in toxic testing. This research focused on the evaluation of cryo-protection on Xenopus embryos. Five solutions (2 different kinds of ethylene glycol, Galactose, DMSO, and PVP) were used as cryo-protection. Twenty embryos were used for each treatment. For each treatment, embryos were placed in a Petri dish containing 8ml of the cryo-protection solution plus one control solution for 5 minutes. . Twenty embryos were placed into 8M of ethylene glycol than replaced into 2M of ethylene glycol. The same amount of embryos was placed into galactose, DMSO and PVP. Then the embryos were placed into plastic straws capped with parafin. Those straws were then placed in a -20[degrees]C freezer until thaw. After 3 days, the embryos were unfrozen by swirling them gently into a water bath for about one minute. They were then placed into a Petri dish containing control solution, and observed. Embryos in the DMSO, galactose, and ethylene glycol lysised. For the PVP, nineteen of those embryos were recognizable but had some deformation. Thirty minutes later, the eggs started to reshape. Some cells were alive but not the embryos. The PVP was seen to be the best cryo-protection but more tests including toxicity testing need to be done. This experiment was supported by the Alabama Academy of Science research award.