Adenovirus vector rescue, production and analysis

Adenoviral vectors are among the oldest and most widely used vectors for gene therapy applications. Compared to other types of vectors, they have many advantages, including the extensive packaging capacity, the ability to transduce a variety of different cell types, and its potential for high-titer preparations. This report focuses on the main steps for production of first-generation adenovirus vectors as well as the introduction and principles of the most important methods to analyze and characterize adenovirus vector preparations.