A Practical Guide to Protein and Peptide Purification for Microsequencing

Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

- Mass spectrophotometric characterization of proteins and peptides

- N-terminal sequencing of proteins with blocked N termini

- Internal amino acid sequence analysis after protease digestion in-gel and on-blot

- Improved microscale peptide purification methods

- Computer analysis of protein sequences

- New protocols tested and refined through everyday use in authors' laboratories

- Updated reference chapter covering all aspects of protein microsequencing