A Survey of Genetic Changes and Search for Sex-Specific Markers by AFLP and SAMPL in a Breeding Program of Chinese Shrimp (Penaeus Chinensis). A Survey of Genetic Changes and Search for Sex-Specific Markers by AFLP and SAMPL in a Breeding Program of Chinese Shrimp (Penaeus Chinensis).

A Survey of Genetic Changes and Search for Sex-Specific Markers by AFLP and SAMPL in a Breeding Program of Chinese Shrimp (Penaeus Chinensis)‪.‬

Journal of Shellfish Research 2004, Dec, 23, 3

Journal of Shellfish Research
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Publisher Description

ABSTRACT Amplified fragment length polymorphism (AFLP) and selective amplified microsatellite polymorphic loci (SAMPL) were used for a survey of genetic changes over three generations and search for sex-specific markers in a breeding program of Chinese shrimp Penaeus chinensis. For genetic survey, a total of 247 and 140 clearly defined bands from 6 AFLP and 4 SAMPL primer sets, respectively, were generated. Both estimated percentage of polymorphic loci (ranging from 41.3% to 47.8%) and average gene diversity (ranging from 0.168 to 0.190) were not significantly different from each other among generation samples, suggesting no significant change at level of genetic variation by AFLP and SAMPL analysis. Despite the frequency change of band-presence allele at all loci over generations showed no clear and traceable patterns, the values of genetic distances and identities between founder stock and sequential generations reflected the accumulation of genetic changes over generations, probably due to isolation and selection. Analysis of molecular variance (AMOVA) and pairwise [[PHI].sub.PT] statistics of AFLP and SAMPL data indicated that significant genetic variation was distributed among generation samples (P 0.01). The use of more variable markers may help further examination of genetic change over generations in more details. Search for sex-specific markers with AFLP and SAMPL loci in this study failed to detect any putative markers, despite 2,110 bands in total were generated through extensive screening with 25 AFLP and 16 SAMPL primer pairs. The inability in detection of sex-specific markers may be due to any of several reasons such as weak correlation between the genotypic and phenotypic sex, high genetic diversity in sex-related regions, or just lack of enough number of loci for screening. KEY WORDS: shrimp, Penaeus chinensis, AFLP, SAMPL, genetic changes, sex-specific markers

GENRE
Science & Nature
RELEASED
2004
December 1
LANGUAGE
EN
English
LENGTH
18
Pages
PUBLISHER
National Shellfisheries Association, Inc.
SELLER
The Gale Group, Inc., a Delaware corporation and an affiliate of Cengage Learning, Inc.
SIZE
212
KB

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