Effect of Different Concentrations of Kinetin on Regeneration of Ten Weeks (Matthiola Incana) (Report‪)‬

Introduction Matthiola incana (Brassicaceae) is an ornamental plant. The Brassicaceae is a fairly large family with many economically important taxa. Ornamental plants are produced mainly for their aesthetic value. The economic value of ornamental plants has significantly been increased worldwide and to be continued 8-10% annually (Jain and Ochatt, 2010). Traditionally, Matthiola incana is propagated via seed. In the field of ornamental plants, tissue culture has allowed mass propagation of superior genotypes and plant improvement, thus enabling the commercialization of healthy and uniform planting material (Winkelmann et al., 2006; Nhut et al., 2006). Plant growth regulators are the most important factors for successful plant regeneration. In tissue culture, cytokinins play a crucial role as promoters of cell division and act in the induction and development of meristematic centers leading to the formation of organs, mainly shoots (Peeters et al., 1991). Kinetin (KIN) has been applied for micropropagation of many ornamental plants (Jain and Ochatt, 2010). In vitro shoot proliferation and multiplication are largely based on media formulations containing cytokinins as a major plant growth regulators (Mamidala and Nanna, 2009; Hoque, 2010). Some reports indicated that the presence of cytokinin in the culture medium helped in the multiplication of shoots (Kumar et al., 2001). Rooting can be initiated by transferring the regenerated shoots to a medium containing only auxins. The best explants for micropropagation of most plants, without any intervening callus phase, are shoot tips and axillary buds (Pati et al., 2005; Hashemabadi and Kaviani, 2010). In general, the number of publications on different aspects of the culture of Matthiola incana with emphasis on somatic explants micropropagation is limited; (Gautam et al., 1983). This paper deals with the effects of KIN on shoot and root regeneration of Matthiola incana.