Each chapter in this book corresponds to a lab in the CHEM 3753: Introduction to Biochemical Methods course at the University of Oklahoma. All of the materials you will need for each lab can be found within its respective chapter. Each chapter will contain a brief introduction; a set of learning objectives; a slide presentation, screencast, or lab demonstration video; the protocol to be followed during your time in the lab; and a set of interactive quiz questions to help you check your understanding as you go. Other interactive features include photos from the lab, links to safety data sheets (SDS), and 3D chemical structures. Components of the book are elaborated upon below. We hope you find this book to be an all-in-one, fun, and engaging learning tool for the biochemical methods course.