Selective Precipitation Based Economical Methods for Purification of Human Serum Albumin from the Out-Dated Blood Bank Samples (Report) Selective Precipitation Based Economical Methods for Purification of Human Serum Albumin from the Out-Dated Blood Bank Samples (Report)

Selective Precipitation Based Economical Methods for Purification of Human Serum Albumin from the Out-Dated Blood Bank Samples (Report‪)‬

Pakistan Journal of Zoology 2010, August 31, 42, 4

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Publisher Description

Byline: M.S. Nadeem, A. Rauf, M. Mustafa, M. Iqbal and A.R. Shakoori - Email: arshak@brain.net.pk Abstract.Based on the studies of solubility of serum albumin in the organic solvents in the presence of trichloroacetic acid and its heat stability in the presence of octanoic acid, two methods have been developed for its purification by selective ammonium sulphate and cold ethanol precipitations. The purification methods used in the present study gave 32% recovery with up to 97% pure final product. The purity and molecular weights of final product was confirmed by SDS-PAGE and gel-exclusion chromatography.

GENRE
Science & Nature
RELEASED
2010
August 31
LANGUAGE
EN
English
LENGTH
7
Pages
PUBLISHER
Asianet-Pakistan
SELLER
The Gale Group, Inc., a Delaware corporation and an affiliate of Cengage Learning, Inc.
SIZE
78
KB
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