Addition of a Homologous Internal Control to a Real-Time PCR Assay for Detection of Bordetella Pertussis (Technical Briefs) Addition of a Homologous Internal Control to a Real-Time PCR Assay for Detection of Bordetella Pertussis (Technical Briefs)

Addition of a Homologous Internal Control to a Real-Time PCR Assay for Detection of Bordetella Pertussis (Technical Briefs‪)‬

Clinical Chemistry 2005, Dec, 51, 12

Clinical Chemistry
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Publisher Description

Pertussis, also called whooping cough, is caused by Bordetella pertussis. The disease may show an atypical course, particularly in neonates and elderly patients. A rapid and safe diagnostic method is thus essential for appropriate treatment and prophylaxis. Culture has been considered the gold standard for detection of B. pertussis, but this method often lacks sensitivity, and a minimum of 4 days may be required to obtain results (1, 2). PCR is a rapid, sensitive, and specific method for the diagnosis of pertussis (3-5). In this study, a new molecular assay was established based on real-time PCR and including a homologous internal control (IC). We evaluated the performance of this assay with a commercially available genomic DNA isolate and with clinical samples.

GENRE
Science & Nature
RELEASED
2005
1 December
LANGUAGE
EN
English
LENGTH
8
Pages
PUBLISHER
American Association for Clinical Chemistry, Inc.
SELLER
The Gale Group, Inc., a Delaware corporation and an affiliate of Cengage Learning, Inc.
SIZE
230.8
KB

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