Comparative Efficacy of Pcr-Based Restriction Fragment Length Polymorphism (RFLP) & Multiplex PCR for Glycoprotein B (Gb) Genotyping of Human Cytomegalovirus (Polymerase Chain Reaction) (Report)
Indian Journal of Medical Research 2007, August, 126, 2
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Publisher Description
Background & objectives: Glycoprotein B (gB), involved in ceil-to-cell transmission of human cytomegalovirus (HCMV), is a critical factor in tissue tropism and viral pathogenesis. The aim of the present study was to compare the efficiency of PCR-based RFLP and multiplex nested PCR for gB gene of HCMV to determine their genotype in clinical specimens from patients with HCMV. Methods: The PCR based RFLP and the multiplex nested PCR were applied on standard strain of HCMV AD169, 4 clinical HCMV isolates and 70 clinical specimens positive for HCMV by pp65 antigenaemia assay or nested PCR for mtr II region or both.
GENRE
Science & Nature
RELEASED
2007
1 August
LANGUAGE
EN
English
LENGTH
14
Pages
PUBLISHER
Indian Council of Medical Research
SELLER
The Gale Group, Inc., a Delaware corporation and an affiliate of Cengage Learning, Inc.
SIZE
231.4
KB
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