APPRAISAL OF SECONDARY METABOLITES IN IN VITRO CULTURES OF Citrullus Colocynthis (L.) Schard (Report‪)‬

Byline: Hina Tanveer, Safdar Ali and Zaheer-ud-Din-Khan ABSTRACT : Plant cell culture systems represent a potential renewable source of valuable medicinal compounds, flavors, fragrances, and colorants, which cannot be produced by microbial cells or chemical synthesis. The present research work was carried out for the analysis of secondary metabolites in callus culture of Citrullus colocynthis (L.) Schard. In vitro grown seedlings were used as explant source. Murashige and Skoog (MS) medium was used as culture medium supplemented with different concentrations and combinations of 2,4-D + Kin mg/L and BAP + NAA mg/L. The combination of BAP 2 mg/L and NAA 3 mg/L was commonly successful in inducing callus in leaf as well as in internode explants while NAA in individual application did not produced good results for callus induction. Laboratory grown seedling parts and the relevant callus cultures raised from different explants were subjected to analysis for secondary metabolites through GC-MS.