USE OF ELECTROPHORETICALLY SEPARATED SERUM PROTEIN FRACTIONS FOR THE DIAGNOSIS OF Cardiomyopathy (Report‪)‬

Byline: Zahid Hussain Siddiqui and Abdul Majeed Cheema ABSTRACT: In an investigation of molecular pathogenesis in cardiovascular diseases, the blood samples of the patients diagnosed for cardiomyopathy (CMP) were obtained from the Punjab Institute of Cardiology, Lahore. Blood samples of the healthy subjects of comparable age group without any history of cardiac ailment were also collected for the control comparisons. The sera of CMP were separated and used for the study of the protein profiles with sodium dodecyle sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in first dimension. Quantification of various protein fractions done by Gene Genius Bio-imaging Gel Documentation System that provide the data of molecular weights and the percent raw volume covered by each of the fractions. The protein fractions that showed significant variation were separated by using the technique of electroblotting and electroelution and run on isoelectric focusing (IEF) in second dimension to determine their isoelectric points.